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KMID : 0363020050350040839
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 4 p.839 ~ p.850
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Effect of titanium surface roughness on adhesion and differentiation of osteoblasts
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김정식
이재관/고성희/엄흥식/장범석
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Abstract
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The success of an implant is determined by its integration into the tissue surrounding the
biomaterial. Surface roughness is considered to influence the behavior of adherent cells. The
aim of this in vitro study was to determine the effect of surface roughness on Saos-2
osteoblast-like cells. Titanium disks blasted with 75 ㎛ aluminum oxide particles and machined
titanium disks were prepared. Saos-2 were plated on the disks at a density of 50,000 cells per
well in 48-well dishes. After 1 hour, 1 day, 6 days cell numbers were counted. One day, 6
days after plating, alkaline phosphatase(ALPase) activity was determined. Compared to
experimental group, the number of cells was significantly higher on control group. The
stimulatory effect of surface roughness on ALPase was more pronounced on the experimental
group than on control group. These results demonstrate that surface roughness alters
proliferation and differentiation of osteoblasts. The results also suggest that implant surface
roughness may play a role in determining phenotypic expression of cells.
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KEYWORD
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Surface roughness, Alkaline phosphatase activity, Implant surface roughness
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